DISTINGUISHING SUBSTRATE-SPECIFIC PHOSPHATASES
نویسندگان
چکیده
منابع مشابه
The Histochemical Demonstration of Secretory Capillaries in the Pancreas with the Aid of Substrate Specific Phosphatases
Staining of the bile canaliculi in the liver of various mammalian species with histochemical techniques for adenosinetriphosphatase and less regularly 5-nucleotidase at pH 7.2 has been previously reported (i-3). When such techniques were applied to the pancreas, marked enzymatic activity was exhibited by the initial portions of the excretory duct system within the centrally located acinar lumen...
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Epidermal growth factor receptor (EGFR) pathway substrate 15 (Eps15) is a newly identified substrate for protein tyrosine phosphatase N3 (PTPN3), which belongs to the FERM-containing PTP subfamily comprising five members including PTPN3, N4, N13, N14, and N21. We solved the crystal structures of the PTPN3-Eps15 phosphopeptide complex and found that His812 of PTPN3 and Pro850 of Eps15 are respon...
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Purple acid phosphatases (PAPs) are dinuclear monoesterases1,2 that are structurally diverse but with a conserved set of ligands to the Fe3+-M2+ metal centers (M2+ ) Fe, Zn, or Mn).3 The identity of the nucleophile and the substrate binding mode have been matters of controversy (Figure 1).1,3-5 In one proposal, substrate binds to the divalent metal and is attacked by a terminal Fe3+-bound hydro...
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Protein tyrosine phosphatases dephosphorylate tyrosine residues of proteins, whereas, dual specificity phosphatases (DUSPs) are a subgroup of protein tyrosine phosphatases that dephosphorylate not only Tyr(P) residue, but also the Ser(P) and Thr(P) residues of proteins. The DUSPs are linked to the regulation of many cellular functions and signaling pathways. Though many cellular targets of DUSP...
متن کاملSubstrate selectivity in the action of alkaline and acid phosphatases.
Alkaline phosphatase of intestinal origin hydrolyzed the S-substituted monoesters of phosphorothioic acid of the type RSP03Na2 (R = -CH2CH2NH2, -CH,CH,NHCOCH,, -CH,COO, or -CH2CHsCOOCzHs) at the S-P bond to yield orthophosphate and the corresponding thioalcohols. The rate of enzymic hydrolysis of cysteamine S-phosphate was measured at pH 9.0 in 0.1 N sodium barbital buffer at different concentr...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1958
ISSN: 0022-1554,1551-5044
DOI: 10.1177/6.4.251